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Published on: January 19, 2024
[Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography]
Yun Ling1, Hong-yu Zhao, Xin-liang Pan
1Division of Shanghan and Jingui, Preclinical Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China.
Objective:
To develop a high-performance liquid chromatographic method for the determination of isoguanosine in Semen Crotonis Tiglii.
Methods:
The determination was done by using reverse-phase high-performance liquid chromatography with a Hedera ODS-2 column (4.6 mm x 250 mm, 5 microm). Elution was employed with the mobile phase of acetonitrile-methanol-water (1:4:95) at flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm and the column temperature was 25 degrees centigrade. Injection volume was 10 microL.
Results:
The linear range of isoguanosine was from 0.161 to 0.967 mg. The correlation coefficient of the calibration curves was 0.999 8. The average recovery rate was 98.78% with relative standard deviation of 0.02%.
Conclusion:
The results show that the method is simple, accurate, and repeatable, and it is suitable for quality control of Semen Crotonis Tiglii.
