Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antitumor Activity of Liposomal Nanoparticles Co-Encapsulating Ceramides and Doxorubicin in In Vitro Nucleolin-Expressing Neuroblastoma Models.

Cells·2026
Same author

Effects of Live and Heat-Treated Bifidobacterium longum CECT 7347 in Adults With Allergic Rhinitis: A Randomised, Double-Blind, Placebo-Controlled Trial.

Allergy·2026
Same author

A Randomised, Double-Blind, Placebo-Controlled Trial of Probiotic and Postbiotic Strains in Healthy Adults with Self-Reported Anxiety: Effects on Mood, Vitality, Quality of Life and Perceived Stress.

Brain sciences·2026
Same author

Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: <i>In vitro</i> Studies Using Lung Cancer Cells.

International journal of nanomedicine·2026
Same author

Biophysical characterisation of mRNA structure and its impact on integrity analysis by liquid chromatography and capillary gel electrophoresis methods.

Journal of chromatography. A·2026
Same author

Cross-presentation of dead cell-associated antigens shapes the neoantigenic landscape of tumor immunity.

Nature immunology·2026

Related Experiment Video

Updated: Jun 18, 2026

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Targeted lipoplexes for siRNA delivery.

Ana Cardoso1, Sara Trabulo, João Nuno Moreira

  • 1Department of Biochemistry, Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal.

Methods in Enzymology
|November 17, 2009
PubMed
Summary

Transferrin-lipoplexes offer a promising method for delivering small interfering RNAs (siRNAs) to cells. This approach enhances gene silencing efficiency for therapeutic applications.

More Related Videos

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Related Experiment Videos

Last Updated: Jun 18, 2026

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • RNA interference (RNAi) is a key mechanism for gene silencing.
  • Effective delivery of small interfering RNAs (siRNAs) is crucial for therapeutic applications.
  • Targeted lipoplexes are explored as delivery vehicles for siRNAs.

Purpose of the Study:

  • To describe the preparation and biocompatibility of transferrin-lipoplexes (Tf-lipoplexes).
  • To evaluate the efficiency of Tf-lipoplexes in delivering siRNAs for gene silencing.
  • To demonstrate the potential of Tf-lipoplexes for therapeutic RNAi applications.

Main Methods:

  • Preparation of Tf-lipoplexes via electrostatic association of transferrin to cationic liposomes.
  • Assessment of siRNA complexation, protection, and intracellular delivery.
  • Evaluation of mRNA and protein knockdown using reporter gene systems (GFP and luciferase).

Main Results:

  • Tf-lipoplexes were successfully prepared and demonstrated biocompatibility.
  • Tf-lipoplexes effectively complexed and protected siRNAs.
  • Efficient intracellular delivery and subsequent gene silencing (mRNA and protein knockdown) were achieved for GFP and luciferase.

Conclusions:

  • Tf-lipoplexes represent an efficient system for targeted siRNA delivery.
  • This method enhances gene silencing mediated by RNA interference.
  • Tf-lipoplexes show promise for in vitro and in vivo therapeutic applications.