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Updated: Jun 18, 2026

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry
Published on: June 10, 2018
Calibration of chromatographic systems for quantitative prediction of chromatography of homopolymers
Alexei A Gorbunov1, Andrey V Vakhrushev, Bernd Trathnigg
1Institute for Highly Pure Biopreparations, 7 Pudozhskaya, 197110 St. Petersburg, Russia. alexeigorbunov@hotmail.com
Abstract:
A method is proposed for obtaining from the experimental data the main parameters, which determine the chromatographic behavior of homopolymers: a dependence of the adsorption interaction parameter on the mobile phase composition, and the pore volume and pore size values. The interaction parameter can be determined by this method in both adsorption, critical, and SEC-type regimes of chromatography. The method is tested in 'mathematical experiments' with theoretical data that mimic experiments, and is applied to calibrate real chromatographic systems. With the obtained calibrations quantitative modeling of chromatograms of PEG samples on two typical ODS columns with different pore size in methanol/water and acetone/water mixed solvents is performed; the simulation results are compared with real chromatograms.
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