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Updated: Jun 18, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
High-resolution low-dose scanning transmission electron microscopy
James P Buban1, Quentin Ramasse, Bryant Gipson
1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, 1 Shields Ave, Davis, CA, USA. james.buban@gmail.com
Abstract:
During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording with a conventional field-emission gun STEM, while maintaining the high-resolution capability of the instrument. Our findings show that a combination of reduced pixel dwell time and reduced gun current can achieve radiation doses comparable to low-dose TEM.
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