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Updated: Jun 18, 2026

Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
Compositional changes in sugarcane bagasse on low temperature, long-term diluted ammonia treatment
Misook Kim1, Giovanna Aita, Donal F Day
1Audubon Sugar Institute, Louisiana State University, Baton Rouge, LA 70776, USA.
Storing sugarcane bagasse with dilute ammonia effectively removes lignin while preserving cellulose. This simple pretreatment enhances bagasse for biofuel and chemical production.
Area of Science:
- Biomass Pretreatment
- Renewable Energy Feedstocks
- Agricultural By-products
Background:
- Sugarcane bagasse is a major lignocellulosic byproduct of the sugar industry.
- Its high cellulose and hemicellulose content makes it a valuable resource for biofuels and chemicals.
- Efficient pretreatment is crucial for unlocking the potential of sugarcane bagasse.
Purpose of the Study:
- To investigate a simple and economical dilute ammonia pretreatment method for sugarcane bagasse storage.
- To evaluate the impact of ammonium hydroxide concentration on biomass composition over time.
- To assess the feasibility of this method for preparing bagasse for further processing.
Main Methods:
- Sugarcane bagasse was stored in 0%, 0.03%, and 0.3% (w/w) ammonium hydroxide solutions.
- Storage occurred in closed bottles at 30°C for 40 days without agitation.
- Samples were analyzed every 10 days for lignin, cellulose, hemicellulose, ammonia, and microbial content.
Main Results:
- Storage at 0.3% ammonium hydroxide for 40 days resulted in 46% lignin removal.
- This condition effectively preserved cellulose (100% retention) and hemicellulose (73% retention).
- Lower concentrations of ammonium hydroxide showed less significant lignin removal.
Conclusions:
- Dilute ammonia pretreatment is a viable and economical method for sugarcane bagasse storage.
- This process facilitates lignin removal while maintaining valuable cellulose and hemicellulose content.
- The optimized pretreatment enhances bagasse suitability for biofuel and biochemical applications.
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