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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...

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Related Experiment Video

Updated: Jun 18, 2026

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

High-resolution atomic force microscopy of DNA.

D V Klinov1, T V Neretina, V V Prokhorov

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. klinov@ibch.ru

Biochemistry. Biokhimiia
|November 18, 2009
PubMed
Summary
This summary is machine-generated.

High-resolution atomic force microscopy now images DNA's fine structure. This advanced technique visualizes the helical motifs of deoxyribonucleic acid (DNA) molecules, including single-stranded DNA.

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) is a powerful tool for nanoscale imaging.
  • Visualizing the fine structure of deoxyribonucleic acid (DNA) at high resolution remains a challenge.

Purpose of the Study:

  • To develop an advanced AFM method for high-resolution DNA imaging.
  • To enable visualization of DNA's detailed structural features, including helical motifs.

Main Methods:

  • Utilized super-sharp probes for enhanced imaging.
  • Employed modified graphite as a substrate for optimal molecule adsorption.
  • Applied high-resolution atomic force microscopy (AFM).

Main Results:

  • Obtained high-resolution images of DNA molecules.
  • Successfully visualized the fine structure, revealing repeated helical motifs.
  • Demonstrated the capability to image individual spread molecules of single-stranded DNA.

Conclusions:

  • The elaborated AFM method provides unprecedented resolution for DNA imaging.
  • This technique facilitates detailed structural analysis of DNA at the molecular level.
  • The method is applicable to both double-stranded and single-stranded DNA visualization.