Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes and plasmid-associated features in KPC-producing Klebsiella pneumoniae bacteraemia.

Tropical medicine and health·2026
Same author

Does the severity of neutropenia affect mortality in bacteremic cancer patients?

BMC infectious diseases·2026
Same author

Whole-protein screening and multi-modal profiling of antigen-specific CD4<sup>+</sup> T cells at single-cell resolution.

Nature communications·2026
Same author

Barriers to Cervical Cancer Screening Follow-up: A Study of an Inpatient Screening Program.

Journal of lower genital tract disease·2026
Same author

Neoadjuvant Fc-enhanced anti-CTLA-4 targets Tregs to augment androgen deprivation in high-risk prostate cancer: A randomized phase I trial.

Cell reports. Medicine·2026
Same author

The path forward for T cell engagers in patients with prostate cancer.

Journal for immunotherapy of cancer·2026

Related Experiment Video

Updated: Jun 18, 2026

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Tc17 CD8 T cells: functional plasticity and subset diversity.

Hung-Rong Yen1, Timothy J Harris, Satoshi Wada

  • 1Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|November 18, 2009
PubMed
Summary

IL-17-secreting CD8 T cells (Tc17) lack direct killing ability but show plasticity. Upon transfer, Tc17 cells convert to IFN-gamma producers, suggesting therapeutic potential.

More Related Videos

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Related Experiment Videos

Last Updated: Jun 18, 2026

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Area of Science:

  • Immunology
  • Cellular Biology

Background:

  • IL-17-secreting CD8 T cells (Tc17) are recognized, yet their functional attributes remain largely uncharacterized.
  • Existing knowledge contrasts Tc17 cells with cytotoxic T cells (Tc1), highlighting differences in lytic function.

Purpose of the Study:

  • To elucidate the functional characteristics and plasticity of IL-17-secreting CD8 T cells (Tc17).
  • To investigate the role of STAT3 in Tc17 cell polarization.
  • To assess the therapeutic potential of Tc17 cells through adoptive transfer studies.

Main Methods:

  • In vitro culture and functional assays of Tc17 cells.
  • STAT3 signaling pathway analysis.
  • Adoptive transfer of purified, antigen-specific Tc17 cells into host models.
  • Assessment of cell phenotype, lytic activity, and cytokine production post-transfer.

Main Results:

  • Tc17 cells demonstrated a lack of in vitro lytic function, distinct from Tc1 cells.
  • STAT3 was identified as crucial for Tc17 polarization both in vitro and in vivo.
  • Adoptive transfer of Tc17 cells led to a phenotypic shift towards IFN-gamma secretion and caused pulmonary pathology.
  • Tc17 cells exhibited greater accumulation compared to Tc1 cells in vivo.

Conclusions:

  • Tc17 cells possess functional plasticity, capable of converting to IFN-gamma-secreting cells.
  • STAT3 is a key regulator in the development and function of Tc17 cells.
  • The plasticity and accumulation of Tc17 cells suggest potential therapeutic applications in diseases requiring IFN-gamma-producing T cells.