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Updated: Jun 18, 2026

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
Published on: September 7, 2012
Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease
Gregory L Szot1, Michael R Lee, Mohammad M Tavakol
1Department of Internal Medicine, Diabetes Center, University of California-San Francisco, San Francisco, CA, USA.
Abstract:
In 2007, the islet community was notified that the collagenase product most commonly used for human islet isolations contained bovine neural tissue contaminants. To minimize this potential hazard, we adapted our human islet processing procedure to use a GMP-manufactured, bovine neural tissue-free collagenase blend. Here, we describe the factors that we consider most important for achieving reproducible and clinically useable islet isolations using this product.

