Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Severe OSA May Be Associated With Endothelial Dysfunction in Patients With Nocturnal Hypertension.

Archivos de bronconeumologia·2026
Same author

Microfluidic patch integrated with cobalt oxide/cobalt phosphate nanozyme for electrochemical lactate sensing at neutral pH.

Talanta·2026
Same author

OSA and Acute Coronary Syndrome Severity: The Potential Role of Coronary Collateral Circulation.

Chest·2026
Same author

Analgesic Effect of a Novel Intravenous Ibuprofen-Low-Dose Tramadol Combination: A Multimodal Approach to Moderate-to-Severe Postoperative Dental Pain.

Pharmaceutics·2025
Same author

A Polydopamine-Based Molecularly Imprinted Electrochemical Sensor for Fentanyl Determination.

ACS omega·2025
Same author

Effect of continuous positive airway pressure on blood pressure in normotensive individuals with obstructive sleep apnoea: a randomised trial.

The European respiratory journal·2025

Related Experiment Video

Updated: Jun 18, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Capillary electrochromatography with packed bead beds in microfluidic devices.

Abebaw B Jemere1, Dolores Martinez, Michael Finot

  • 1National Institute for Nanotechnology, National Research Council Canada, Edmonton, Alberta, Canada.

Electrophoresis
|November 20, 2009
PubMed
Summary

Microchip electrochromatography columns efficiently separate proteins and peptides using size-exclusion and ion-exchange methods. This technology shows promise for rapid drug solubility testing in pharmaceutical development.

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jun 18, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Microchip-based chromatography offers miniaturized and efficient separation solutions.
  • Electrochromatography (EC) combines electroosmotic flow with chromatographic separation principles.
  • Development of packed microcolumns is crucial for advancing microchip EC applications.

Purpose of the Study:

  • To develop and characterize microchip-based bead-packed columns for electrochromatography.
  • To evaluate the separation performance of these columns for proteins and peptides.
  • To demonstrate the utility of microchip EC in pharmaceutical analysis.

Main Methods:

  • Fabrication of microchip columns packed with various stationary phases.
  • Electrochromatographic separations using size-exclusion and ion-exchange mechanisms.
  • Analysis of fluorescently labeled proteins (FITC-IgG, FITC-insulin) and peptides.
  • Application of a reverse-phase column for drug solubility testing.

Main Results:

  • Baseline resolution of FITC-IgG and FITC-insulin in <10 s using size-exclusion EC.
  • High efficiencies achieved: up to 139,000 plates/m (size-exclusion) and 400,000 plates/m (ion-exchange).
  • Baseline resolution of three labeled peptides in <40 s using strong cation-exchange EC.
  • Low relative standard deviation (<3%) for analyte retention times in both modes.
  • Demonstrated utility in semiquantitative evaluation of pharmaceutical formulations.

Conclusions:

  • Microchip-based bead-packed columns provide high-efficiency and rapid separations for biomolecules.
  • Size-exclusion and ion-exchange electrochromatography modes are effective on these microchips.
  • The microfluidic chip-based EC approach is suitable for drug development applications, including solubility testing.