Jove
Visualize
Contact Us

Related Concept Videos

Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Recombinant DNA01:09

Recombinant DNA

Overview
Recombinant DNA01:09

Recombinant DNA

Overview
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shared neural representation between motor execution and kinesthetic imagery in the primary motor cortex.

Imaging neuroscience (Cambridge, Mass.)·2026
Same author

Insights into adaptation mechanisms and survival strategies of Pseudomonas chlororaphis subsp. aurantiaca: A comparative proteomic study under PFOA exposure.

Ecotoxicology and environmental safety·2026
Same author

MLP-PG1 identified from <i>Cucurbita pepo</i> confers drought tolerance via ABA-mediated signaling system.

Physiology and molecular biology of plants : an international journal of functional plant biology·2026
Same author

Olfactory Bulb Volume and Function Recovery in Eosinophilic Chronic Rhinosinusitis.

The Laryngoscope·2026
Same author

Clinical characteristics and triage acuity of patients at Kanazawa university hospital after the 2024 Noto Peninsula Earthquake.

Injury·2026
Same author

Extracellular secretion of major latex-like proteins related to the accumulation of the hydrophobic pollutants dieldrin and dioxins in Cucurbita pepo.

Plant physiology and biochemistry : PPB·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 18, 2026

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
07:36

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Published on: May 16, 2020

Designed recombinant transcription factor with antibody-variable regions.

Keiko Gion1, Yuki Sakurai, Akito Watari

  • 1Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.

Analytical Chemistry
|November 21, 2009
PubMed
Summary

Researchers developed a novel inducible transcription factor system. This system uses antibody fragments to control gene expression, allowing custom inducer selection for applications like gene therapy.

More Related Videos

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Related Experiment Videos

Last Updated: Jun 18, 2026

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
07:36

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Published on: May 16, 2020

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Immunology

Background:

  • Existing recombinant transcription factors lack selectable inducers.
  • Controlling gene expression with specific molecules is crucial for various biotechnological applications.

Purpose of the Study:

  • To create a novel, inducible transcription factor system selectable by a specific inducer molecule.
  • To demonstrate the functionality of this system using antibody-derived transcription factors.

Main Methods:

  • Constructed recombinant transcription factors by fusing antibody variable fragments (Fvs) with DNA-binding domains (LexA) and transactivation domains (VP16).
  • Engineered constructs included nuclear localization signals (NLS) for enhanced function.
  • Tested induction of beta-galactosidase (lacZ) expression in yeast cells in the presence of specific inducers (bisphenol A or PCB derivatives).

Main Results:

  • Successfully created a functional transcription factor system inducible by bisphenol A (BPA) using Fvs from a BPA-specific monoclonal antibody (mAb).
  • Demonstrated significantly increased lacZ activity in response to a polychlorinated biphenyl (PCB) derivative when using Fvs from a PCB-specific mAb.
  • Optimized construct design with NLS and specific fusion orientations enhanced gene induction.

Conclusions:

  • The developed Fv-based transcription factor system offers a novel method for inducible gene expression.
  • This system allows for the selection of specific molecules as inducers by preparing monoclonal antibodies against them.
  • Potential applications in gene therapy and other fields requiring precise gene regulation.