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[Nicotine affects biological properties of the human osteosarcoma cell line MG63]
Bing Bai1, Li-fang Zhong, Jing-tao Zhu
1Dept. of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, China.
Objective:
To evaluate the effect of nicotine on cell proliferation.
Methods:
The cells were cultured with DMEM medium containing 10% fetal bovine serum with 0, 1 x 10(-4) mol x L(-1), and 1 x 10(-3) mol x L(-1) nicotine for up to 3, 5, 7, 10, 14 days. The cell proliferation was evaluated by MTT. The alkaline phosphatase (ALP) activity was estimated by PNPP. The expression of collagen type I (COL1) and osteocalcin (OCN) were estimated by reverse transcription-polymerase chain reaction (RT-PCR).
Results:
Nicotine suppressed the cell proliferation. ALP activity increased to peak on 10 days in control and 1 x 10(-4) mol x L(-1) nicotine. COL1 expression increased to peak on 10 days in control and 1 x 10(-4) mol x L(-1) nicotine, but there was decreased to the minimum on 10 days and increased on 14 days in 1 x 10(-3) mol x L(-1) nicotine. OCN expression increased to peak on 10 days in control, and increased in 1 x 10(-4) mol x L(-1) nicontine from 3 days to 14 days, but there wasn't significant change in 1 x 10(-3) mol x L(-1) nicontine.
Conclusion:
Nicotine suppresses osteogenesis through a decrease in ALP and COL1 production by osteoblasts.
