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[Differential proteomics analysis of dental pulp cell induced by recombinant human interleukin-1beta]
Shi-liang Guo1, Ying-li Zhang, Yang Huang
1Dept. of Endodontics, College of Stomatology, Jilin University, Changchun 130021, China.
Objective:
To compare the proteomics change of human dental pulp cells induced by recombinant human interleukin-1beta (rhIL-1beta).
Methods:
The dental pulp cell entire protein was separated by a two-dimensional electrophoresis (2-DE) technique. The rhIL-1beta induction and the normal dental pulp cell protein 2-DE atlas were established. Difference expression protein was confirmed by ImageMaster 2D Elite 5.0 software analysis. To identify differentially expressed proteins spot by matrix-assisted laser desorption/ionization time of flight mass spectrometry, and get peptide mass fingerprinting.
Results:
Comparing the two groups of protein 2-DE atlas, 39 protein spots were obviously different. Including 15 points in the induction of protein expression were higher, 13 new protein spots, 7 protein points expressions were lower, there were only four points in the control group. After mass spectra identification, 10 protein spots were confirmed at last.
Conclusion:
Pulp cells to rhIL-1beta responsiveness is a very complex process, which involve a variety of protein molecules, rhIL-1beta related 10 protein spots have been identified in the dental pulp cell for the first time. To explore pulpitis's early response mechanism provides a new clue and ideas.
