Related Experiment Video
Updated: Jun 18, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
[Present and future of reverse genetics of rotavirus]
Koki Taniguchi1, Satoshi Komoto, Jun Sasaki
1Department of Virology and Parasitology, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. kokitani@fujita-hu.ac.jp
Abstract:
Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.
Related Concept Videos
Microorganisms in Medicine and Therapeutics
Retroviruses
Retrovirus Life Cycles

