Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association of Multimodal Intracoronary Imaging-Derived Plaque Morphology with the Pathophysiological Disease Patterns Assessed by the Quantitative Flow Ratio-Pullback Pressure Gradient.

Journal of atherosclerosis and thrombosis·2026

Same author

Comprehensive, High-Spatiotemporal-Resolution Intravital Two-Photon Imaging of the Mouse Conventional Outflow Pathway: Green Intravital Imaging.

Investigative ophthalmology & visual science·2026

Same author

Determinants of myocardial extracellular volume fraction derived from coronary CT angiography in patients with chronic coronary syndrome.

Journal of cardiovascular computed tomography·2026

Same author

Comprehensive Endotyping of Patients With Angina and Nonobstructive Coronary Arteries: A Report From the J-CMD.

Journal of the American Heart Association·2026

Same author

Identification of near-infrared spectroscopy-defined lipid content within or adjacent to calcified coronary plaques using optical coherence tomography.

Atherosclerosis·2026

Same author

Apocrine Ductal Carcinoma <i>in situ</i> Ex Pleomorphic Adenoma of the Breast: A Rare Case Report.

Surgical case reports·2026

Same journal

[The history of HIV].

Uirusu·2026

Same journal

[The multidisciplinary study, "Aerosol virology/Aerovirology"-A new frontier].

Uirusu·2026

Same journal

[How viruses hitch a ride on pollen to infect plants].

Uirusu·2026

Same journal

[Study on the pathogenicity and tropism of positive-strand RNA viruses].

Uirusu·2025

Same journal

[Elucidation of virus-host interaction using animal models towards vaccine development].

Uirusu·2025

Same journal

[Epidemiology of measles in Japan].

Uirusu·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 16, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

[Exploration and Functional Analysis of Epstein-Barr Virus Pathogenic Factors Using a Multidimensional Approach].

Takahiro Watanabe¹

¹Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

|September 28, 2025

Summary

This summary is machine-generated.

Epstein-Barr virus (EBV) research is advancing rapidly. New tools like CRISPR/Cas9 and humanized mice models are helping scientists understand EBV

More Related Videos

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Related Experiment Videos

Last Updated: Jan 16, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Area of Science:

Virology
Oncology
Immunology

Background:

Epstein-Barr virus (EBV) infects over 90% of adults, establishing lifelong latent infections.
EBV is linked to various cancers (lymphomas, nasopharyngeal carcinoma, gastric cancer) and autoimmune diseases.
Historical research faced limitations due to challenges in viral culture and animal models.

Purpose of the Study:

To review the evolution of techniques for generating recombinant Epstein-Barr viruses (EBVs).
To discuss advancements in in vivo modeling for EBV research.
To highlight contributions to tools for studying EBV-associated tumorigenesis.

Main Methods:

Whole-genome cloning using bacterial artificial chromosomes (BACs).
Gene editing via CRISPR/Cas9 technology.
Development and application of in vivo models, including humanized mice.

Main Results:

Recent technological advances have accelerated the understanding of EBV's life cycle and oncogenic mechanisms.
New tools enable functional genetic analysis of EBV.
Improved models facilitate research into EBV-associated cancers.

Conclusions:

Technological progress is crucial for overcoming past limitations in EBV research.
Advanced recombinant EBV generation and in vivo models are essential for future discoveries.
These tools are vital for investigating EBV's role in tumorigenesis and disease.