Related Experiment Video

Updated: Jun 18, 2026

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Electron transfer dissociation facilitates sequencing of adenosine diphosphate-ribosylated peptides

Barry M Zee, Benjamin A Garcia

    Analytical Chemistry
    |November 26, 2009
    PubMed

    More Related Videos

    3' End Sequencing Library Preparation with A-seq212:01

    3' End Sequencing Library Preparation with A-seq2

    Published on: October 10, 2017

    Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA12:35

    Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

    Published on: November 14, 2017

    Related Concept Videos

    Maxam-Gilbert Sequencing01:05

    Maxam-Gilbert Sequencing

    In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
    Challenges of the Maxam-Gilbert Method
    The...
    RNA-seq03:21

    RNA-seq

    RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
    Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
    Sanger Sequencing01:57

    Sanger Sequencing

    DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...