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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Functional and Structural Alterations Associated with Dehydration of Lactic Acid Bacteria
Nelson Romano1, Monica Marro2, Maria Marsal3
1Center for Research and Development in Food Science and Technology, La Plata, Argentina.
Abstract:
The dehydration of lactic acid bacteria for technological purposes induces multilevel damage to bacterial cells. This chapter provides protocols for analyzing functional and structural alterations following the dehydration of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 using vacuum drying technology. Protocols involving conventional microbiological assays (plate counting, metabolic activity), fluorescence (membrane integrity), and Raman spectroscopies (DNA conformational analysis) are presented, along with experimental tips and suggestions.
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