Fast interrogation of intestinal organ-on-chip devices through inverted single-plane illumination microscopy (iSPIM)...

We have developed an inverted single-plane illumination microscope adapted for the interrogation of intestinal epithelium organ-on-chip devices. In this kind of system, samples are normally scanned in a slow fashion through motorized stages. Here, we have engineered a faster sample remote focusing scan mode by the use of an electro-tunable lens synchronized with Galvo mirrors, overcoming some limitations of previous systems. In addition, we have also engineered and optimized scaffolds recapitulating the 3D topography of the intestinal epithelium. Finally, we evaluated the performance of the system using fixed and in-vitro intestinal epithelium organ-on-chip devices, demonstrating the possibility of live imaging for several hours without distorting sample evolution.