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Related Concept Videos

Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Proteoglycans01:05

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...

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Related Experiment Video

Updated: Jun 18, 2026

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Glycan array: a powerful tool for glycomics studies.

Chi-Hui Liang1, Chung-Yi Wu

  • 1The Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115, Taiwan. jamesl@gate.sinica.edu.tw

Expert Review of Proteomics
|November 26, 2009
PubMed
Summary
This summary is machine-generated.

Glycan arrays offer high-throughput analysis of carbohydrate interactions with biological targets, advancing glycomics and disease understanding. This review highlights recent innovations and future trends in glycan array technology.

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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

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Related Experiment Videos

Last Updated: Jun 18, 2026

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
11:08

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Area of Science:

  • Carbohydrate Chemistry
  • Glycobiology
  • Biotechnology

Background:

  • Glycan arrays are crucial for studying carbohydrate interactions with biological molecules like proteins and viruses.
  • This technique effectively mimics cell-cell interface interactions, making it ideal for glycomics.
  • Understanding these interactions is vital for deciphering disease-related glycobiology.

Purpose of the Study:

  • To review recent advancements in glycan array fabrication and application.
  • To explore new platforms providing novel biological insights.
  • To forecast future trends and address challenges in glycan array technology.

Main Methods:

  • High-throughput screening using immobilized carbohydrate structures (glycans).
  • Fabrication of novel glycan arrays with improved methods.
  • Utilizing new platforms for data acquisition and analysis.

Main Results:

  • Recent advances include improved fabrication techniques and new biological information platforms.
  • Glycan arrays provide deeper insights into disease mechanisms.
  • The technology is evolving with potential for further applications.

Conclusions:

  • Glycan arrays are powerful tools for glycomics and understanding biological interactions.
  • Continued innovation in fabrication and platforms will enhance their utility.
  • Addressing current challenges will further solidify glycan arrays as a key technology in glycobiology.