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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Single molecule immunoassay on plasmonic platforms.

R Luchowski1, E G Matveeva, T Shtoyko

  • 1Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology & Immunology, UNTHSC, Fort Worth, TX 76107, USA. rluchows@hsc.unt.edu

Current Pharmaceutical Biotechnology
|November 26, 2009
PubMed
Summary
This summary is machine-generated.

We enhanced near-infrared (NIR) fluorescent label SeTau-665 performance using a silver plasmonic platform. This boosts brightness up to 400x for improved single molecule detection and sensing limits.

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Area of Science:

  • Plasmonics
  • Fluorescence Spectroscopy
  • Nanomaterials

Background:

  • Near-infrared (NIR) fluorescent labels are crucial for sensitive biological imaging.
  • Plasmonic nanostructures can enhance fluorescence properties of nearby emitters.
  • Self-assembled colloidal structures (SACS) of silver offer a tunable plasmonic platform.

Purpose of the Study:

  • To investigate the photophysical properties of the SeTau-665 fluorescent label on a silver SACS plasmonic platform.
  • To evaluate the potential of this platform for enhancing fluorescence-based assays.
  • To assess the impact of plasmonic interactions on SeTau-665 brightness and lifetime.

Main Methods:

  • Fabrication of a silver SACS plasmonic platform on a silver film.
  • Performance of SeTau-665 immunoassay on the plasmonic platform and a control glass slide.
  • Characterization of fluorescence properties (brightness, lifetime) using ensemble and single-molecule measurements.

Main Results:

  • SeTau-665 exhibited significantly altered fluorescence properties on the plasmonic platform due to plasmonic interactions.
  • Average brightness of SeTau-665 increased approximately 70-fold in ensemble measurements.
  • Single-molecule measurements revealed fluorescence enhancements up to 400-fold at plasmonic 'hot spots', correlated with decreased fluorescence lifetime.

Conclusions:

  • The silver SACS plasmonic platform substantially enhances the brightness of the SeTau-665 NIR fluorescent label.
  • The observed fluorescence enhancement enables reduced excitation power, leading to lower background and improved photostability.
  • This approach holds significant potential for improving single-molecule detection sensitivity and lowering detection limits in sensing devices.