Related Experiment Video
Updated: Jun 18, 2026
07:53
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Published on: March 28, 2008
Identifying competitive protein antagonists for F-actin with reverse-phase high-performance liquid chromatography
Jeffrey W Brown1, C James McKnight
1Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA.
Analytical Biochemistry
|November 26, 2009
Abstract
None:
F-actin binding constants are traditionally determined by centrifugal cosedimentation with actin microfilaments, where bound protein is separated from actin with SDS-PAGE and quantitated using densitometry. Here, we demonstrate that UV quantitation of reverse-phase HPLC-separated proteins provides increased accuracy and sensitivity, can be fully automated, and allows one to perform F-actin competition assays on similar sized proteins.
