Related Experiment Video
Updated: Jun 18, 2026

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
Published on: April 3, 2019
MALDI-TOF mass spectrometry: a powerful tool to study the internalization of cell-penetrating peptides
Soline Aubry1, Baptiste Aussedat, Diane Delaroche
1Université Pierre et Marie Curie, 4 place Jussieu, 75005 Paris, France.
Abstract:
This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms.
More Related Videos
11:25Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
Published on: December 26, 2013
07:49Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
Related Concept Videos
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry
Rapid Identification of Pathogens
Matrix-Assisted Laser Desorption Ionization (MALDI)