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Is voided urine suitable for flow cytometric DNA analysis?
K Mellon1, B K Shenton, D E Neal
1Department of Urology, Freeman Hospital, Newcastle upon Tyne.
British Journal of Urology
|January 1, 1991
Summary
Bladder washings provide superior cellular material for flow cytometric DNA analysis compared to voided urine. Voided urine specimens yielded significantly fewer satisfactory DNA histograms, limiting its routine clinical use.
Area of Science:
- Urology
- Oncology
- Cell Biology
Background:
- Bladder washings are a common source for flow cytometric DNA analysis.
- Voided urine is a potential alternative for obtaining cellular material.
Purpose of the Study:
- To evaluate the suitability of voided urine for flow cytometric DNA analysis.
- To compare the quality of specimens from voided urine versus bladder washings.
Main Methods:
- Comparative analysis of flow cytometric DNA analysis results.
- Examination of 53 voided urine specimens and 109 bladder washing samples.
Main Results:
- Only 45% of urine specimens yielded satisfactory DNA histograms.
- Bladder washings achieved a 93% satisfactory rate.
- Specimen quality difference was independent of tumor presence.
Conclusions:
- Bladder washings are superior for flow cytometric DNA analysis.
- Voided urine analysis has deficiencies for routine clinical application.