Related Experiment Video
Updated: Jun 18, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Published on: September 1, 2019
The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating
Kazuyoshi Kohu1, Hidetaka Ohmori, Won Fen Wong
1Institute of Development, Aging and Cancer, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Abstract:
Recently, it was reported that the expression of Runt-related transcription factor 3 (Runx3) is up-regulated in CD4(+) helper T cells during Th1 cell differentiation, and that Runx3 functions in a positive feed-forward manner with the T-box family transcription factor, T-bet, which is a master regulator of Th1 cell differentiation. The relative expression levels of IFN-gamma and IL-4 are also regulated by the Th2-associated transcription factor, GATA3. Here, we demonstrate that Runx3 was induced in Th2 as well as Th1 cells and that Runx3 interacted with GATA3 and attenuated GATA3 transcriptional activity. Ectopic expression of Runx3 in vitro in cultured cells or transgenic expression of Runx3 in mice accelerated CD4(+) cells to a Th1-biased population or down-modulated Th2 responses, in part by neutralizing GATA3. Our results suggest that the balance of Runx3 and GATA3 is one factor that influences the manifestation of CD4(+) cells as the Th1 or Th2 phenotypes.
Related Concept Videos
TGF - β Signaling Pathway
Master Transcription Regulators
Master Transcription Regulators
General Transcription Factors
RNA Polymerase II Accessory Proteins
Co-activators and Co-repressors
