Related Experiment Video
Updated: Jun 18, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
[Adhesive properties of Pragia fontium lipopolysaccharides]
Abstract:
Effect of lipopolysaccharides (LPS) and their structure components (lipid A, oligosaccharide core, and O-specific polysaccharides) from 8 strains of P. fontium on the adhesion process have been investigated for the first time. It was demonstrated, that LPS and their structural components may compete with E. coli adhesins and phytohemagglutinin under binding with receptors on the surface of the rabbit erythrocytes. An analysis of different structural components influence indicated, that in comparison with O-specific polysaccharides and core oligosaccharide lipids A of all investigated strains of P. fontium displayed the highest inhibitory action on the adhesion process. It was shown that there existed certain dependence between LPS composition and their effects on the adhesion process. LPS, obtained from P. fontium strains grown at different temperatures (36, 25, 4 degrees C) and characterized by various quantitative and qualitative monosaccharide and fatty acid composition, demonstrated different adhesion properties.
Related Concept Videos
Formation of Lipopolysaccharides
Surface Appendages of Archaea
Outer Layers of the Cell Envelope
Biosynthesis of Lipids
Archaeal Cell Wall

