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Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
Hydroxyapatite particles as drug carriers for proteins.
Keishiro Tomoda1, Hidehiko Ariizumi, Takatomo Nakaji
1Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Colloids and Surfaces. B, Biointerfaces
|November 27, 2009
Summary
Hydroxyapatite (HA) particles were prepared and optimized for protein drug delivery. Controlling experimental conditions, like EDTANa(2) concentration, influences HA particle properties and protein adsorption for sustained release.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Hydroxyapatite (HA) is a biocompatible and biodegradable material found in bone and teeth.
- HA exhibits high affinity for proteins, making it a promising candidate for drug carriers.
- Controlling HA particle properties is crucial for optimizing protein adsorption and release kinetics.
Purpose of the Study:
- To prepare hydroxyapatite (HA) particles using various methods.
- To investigate the influence of experimental conditions on HA particle characteristics, including shape, size, and protein adsorption capacity.
- To evaluate the adsorption and sustained release of proteins from HA particles.
Main Methods:
- HA particles were synthesized via solvent diffusion, sintering of porous granules, and homogeneous precipitation.
- Particle morphology and size were controlled by adjusting temperature, reaction time, and additive concentrations (urea, EDTANa(2)).
- Adsorption and release of Bovine Serum Albumin (BSA) and Lysozyme Hydrochloride (LSZ) were quantified using X-ray powder diffraction and release profile analysis.
Main Results:
- Homogeneous precipitation yielded small, spherical, well-dispersed HA particles.
- EDTANa(2) concentration significantly impacted particle size and surface characteristics, affecting protein adsorption.
- BSA (negatively charged) and LSZ (positively charged) showed differential adsorption based on HA surface charge and particle size.
- Sustained release of both BSA and LSZ from HA particles was observed over a two-week period.
Conclusions:
- The study successfully prepared and characterized HA particles for protein drug delivery.
- Optimizing HA particle synthesis through controlled experimental conditions, particularly EDTANa(2) concentration, is key to tailoring adsorption and release profiles.
- HA particles demonstrate potential for sustained delivery of protein therapeutics, with adsorption influenced by protein charge and particle surface properties.
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