Related Experiment Video
Updated: Jun 18, 2026
04:29
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
Published on: July 1, 2022
Isolation of common dendritic cell progenitors (CDP) from mouse bone marrow
Nobuyuki Onai1, Markus G Manz, Michael A Schmid
1Department of Biodefense Research, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2009
Summary
Researchers identified a common progenitor for plasmacytoid dendritic cells (pDCs) and conventional dendritic cells (cDCs). This common clonogenic progenitor (CDP) generates both cell types, proving a shared developmental pathway in lymphoid organs.
Area of Science:
- Immunology
- Developmental Biology
- Hematopoiesis
Background:
- Dendritic cells (DCs) are crucial immune cells in lymphoid organs, categorized into plasmacytoid DCs (pDCs) and conventional DCs (cDCs).
- The developmental relationship and potential common progenitor for pDCs and cDCs have remained an area of investigation.
Purpose of the Study:
- To identify and characterize a common progenitor capable of differentiating into both pDCs and cDCs.
- To investigate the developmental pathway from hematopoietic stem cells to mature DCs.
Main Methods:
- Utilized antibodies targeting c-kit, Flt3, and M-CSFR to isolate progenitor populations from mouse bone marrow.
- Performed in vitro differentiation assays and in vivo cell transfer experiments in irradiated and steady-state mice.
- Employed single-cell analysis to assess the clonal potential of identified progenitors.
Main Results:
- Identified a common clonogenic progenitor (CDP) in mouse bone marrow.
- CDPs demonstrated the capacity to generate both pDCs and cDCs in vitro and in vivo.
- Single-cell analysis confirmed the existence of progenitors within the CDP population that give rise to both pDC and cDC lineages.
Conclusions:
- The study provides evidence for a common developmental pathway for a subset of pDCs and cDCs.
- The identified CDP represents a crucial link in the differentiation hierarchy of dendritic cells.
