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Updated: Jun 18, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Protein oxidation biomarkers in plasma of type 2 diabetic patients
Kanti Bhooshan Pandey1, Neetu Mishra, Syed Ibrahim Rizvi
1Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Objectives:
To evaluate oxidative stress and the extent of oxidation of plasma proteins in type 2 diabetic patients.
Design And Methods:
Study was carried out on blood from 31 diabetic patients of both sexes (mean age = 58 + or - 7; duration of diabetes 12 + or - 5 years) and healthy age and sex matched normal subjects. Biomarkers of protein oxidation; plasma protein carbonyls (PCO), advanced oxidation protein products (AOPPs) and -SH group and free radical scavenging capacity of plasma was measured.
Results:
PCO and AOPPS levels were significantly (P<0.005) higher in diabetic patients in comparison to healthy volunteers. Reduced free radical scavenging capacity (P<0.001) and -SH group (P<0.05) was observed in plasma of type 2 diabetic patients.
Conclusions:
Our data suggest that diabetics are susceptible to protein oxidation. Oxidative modulation of proteins due to reduced radical scavenging activity of plasma patients may be one of the reasons of altered physiological processes in type 2 diabetic patients.
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