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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 18, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

High-throughput single nucleotide polymorphism genotyping using nanofluidic Dynamic Arrays.

Jun Wang1, Min Lin, Andrew Crenshaw

  • 1Fluidigm Corporation, South San Francisco, CA, USA. jun.wang@fluidigm.com

BMC Genomics
|December 1, 2009
PubMed
Summary
This summary is machine-generated.

The Dynamic Array system offers efficient, cost-effective medium-throughput genotyping for thousands of samples. This nanofluidic platform achieves high accuracy and call rates, ideal for genetic association studies.

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Last Updated: Jun 18, 2026

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Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Area of Science:

  • Genetics
  • Genomics
  • Biotechnology

Background:

  • Single nucleotide polymorphisms (SNPs) are crucial genetic markers for disease locus mapping and association studies.
  • High-throughput, cost-effective genotyping systems are needed for large sample sets (thousands of individuals).
  • Flexible, fast, and accurate genotyping methods are essential, especially for non-model organisms lacking commercial assays.

Purpose of the Study:

  • To evaluate a nanofluidic Integrated Fluidic Circuit (IFC)-based genotyping system, the Dynamic Array, for medium-throughput multiplexing.
  • To assess the system's performance in terms of call rates, accuracy, and cost-effectiveness for large-scale genotyping.

Main Methods:

  • Utilized a nanofluidic Integrated Fluidic Circuit (IFC) Dynamic Array system for genotyping.
  • Genotyped 994 human DNA samples across 47 different single nucleotide polymorphism (SNP) assays.
  • Employed nanoliter volumes of reagents for the assays.

Main Results:

  • Achieved call rates exceeding 99.5% and call accuracies surpassing 99.8%.
  • Demonstrated a simple experimental setup with a time-to-result of approximately 3 hours per sample.
  • The Dynamic Array proved to be a formidable genotyping platform for medium-throughput applications.

Conclusions:

  • The Dynamic Array is an excellent system for medium-throughput genotyping (30-300 SNPs), offering ease of use, rapid throughput, and high accuracy.
  • The system's high call rates and accuracy are particularly valuable for validating and replicating genome-wide association (GWA) studies.
  • This platform provides a cost-effective solution for large-scale genotyping needs in both model and non-model systems.