Related Experiment Video
Updated: Jun 18, 2026

08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
Infectious disease diagnostic assays
1Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1678, USA. michaellappin@colostate.edu
Topics in Companion Animal Medicine
|December 1, 2009
Summary
This review updates common infectious disease assays for dogs and cats. Veterinarians can use molecular diagnostics to manage infectious diseases, but assay accuracy varies, impacting patient care.
Area of Science:
- Veterinary Medicine
- Infectious Diseases
- Diagnostic Assays
Background:
- Dogs and cats frequently encounter infectious disease agents.
- Accurate diagnosis is crucial for effective treatment and disease management in small animals.
Purpose of the Study:
- To provide an update on commonly used infectious disease assays in small animal practice.
- To discuss the utility and limitations of molecular diagnostic assays in veterinary medicine.
Main Methods:
- Review of current literature on infectious disease assays for companion animals.
- Analysis of the application of pathogen-specific molecular diagnostic assays.
Main Results:
- Molecular diagnostic assays can aid veterinarians in managing infectious diseases.
- Assay results can be positive in healthy animals or falsely negative in ill animals.
- The predictive values of these assays vary depending on the specific infectious agent.
Conclusions:
- Veterinarians must carefully interpret infectious disease assay results.
- Understanding assay limitations is essential for accurate diagnosis and patient management.
- Further research may improve the reliability and predictive value of diagnostic assays.
Related Concept Videos
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

