Expression profile of Notch-1 in mechanically overloaded plantaris muscle of mice
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Updated: Jun 18, 2026
In vivo Measurement of Knee Extensor Muscle Function in Mice
Published on: March 4, 2021
Mai Akiho1, Hiroyuki Nakashima, Munehiro Sakata
1Research Center for Physical Fitness, Sports and Health, Toyohashi, University of Technology, 1-1 Hibarigaoka, Tenpaku-cho, Toyohashi, 441-8580, Japan.
Aim:
We investigated the expression pattern of Notch-1 in normal and hypertrophied plantaris muscle of mice.
Main Methods:
We performed immunofluorescence of both Notch-1 and the Notch-1-linking molecules.
Key Findings:
Immunofluorescence labeling revealed Notch-1 protein in Pax7-positive satellite cells during days 2-6. We observed clear co-localization between Notch-1 and myogenin (4.9+/-1.3%) in the hypertrophied muscle at 4days. Several mononuclei (possibly satellite cells) possessed both Notch-1 and Foxo1 in the plantaris muscle subjected to mechanical overloading (4.1+/-1.2%).
Significance:
Notch-1 may play an important role in the maintenance of quiescent satellite cells.
