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Related Experiment Video

Updated: Jun 18, 2026

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
11:53

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Published on: March 18, 2015

Nongenetic method for purifying stem cell-derived cardiomyocytes.

Fumiyuki Hattori1, Hao Chen, Hiromi Yamashita

  • 1Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan.

Nature Methods
|December 1, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to purify stem cell-derived cardiomyocytes using a fluorescent dye and cell sorting. This technique ensures high purity and safety for future therapeutic applications.

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High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
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High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

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Related Experiment Videos

Last Updated: Jun 18, 2026

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
11:53

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Published on: March 18, 2015

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization
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Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

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High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

Area of Science:

  • Stem cell biology
  • Cardiovascular research
  • Cell purification technologies

Background:

  • Pluripotent stem cell (PSC)-derived cardiomyocytes are promising for regenerative medicine.
  • Purification of these cells is challenging due to the lack of specific markers.
  • Eliminating undifferentiated cells is crucial for safe and effective applications.

Purpose of the Study:

  • To develop an efficient and specific method for purifying pluripotent stem cell-derived cardiomyocytes.
  • To assess the safety and efficacy of purified cardiomyocytes in transplantation models.

Main Methods:

  • Utilized tetramethylrhodamine methyl ester perchlorate, a mitochondrial fluorescent dye, for selective cell labeling.
  • Employed fluorescence-activated cell sorting (FACS) for enrichment of labeled cardiomyocytes.
  • Evaluated teratoma formation after transplantation into testes.
  • Assessed cardiomyocyte survival in immunodeficient mouse hearts after aggregate formation.

Main Results:

  • Tetramethylrhodamine methyl ester perchlorate selectively labeled cardiomyocytes from various species (rat, mouse, marmoset, human) and developmental stages.
  • Fluorescence-activated cell sorting achieved >99% purity of cardiomyocytes.
  • Purified cardiomyocytes did not induce teratoma formation upon transplantation.
  • Homophilic cell-cell adhesion-mediated aggregate formation enhanced cardiomyocyte survival in vivo.

Conclusions:

  • A novel, efficient, and specific method for purifying pluripotent stem cell-derived cardiomyocytes has been established.
  • This purification strategy enhances the safety profile by eliminating undifferentiated cells and preventing teratoma formation.
  • Improved in vivo survival of purified cardiomyocytes supports their therapeutic potential.