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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 18, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Inertial microfluidics for sheath-less high-throughput flow cytometry.

Ali Asgar S Bhagat¹, Sathyakumar S Kuntaegowdanahalli, Necati Kaval

¹Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.

Biomedical Microdevices

|December 1, 2009

Summary

This summary is machine-generated.

This study introduces a low-cost, portable flow cytometry system using microfluidics for accessible single-cell analysis. The on-chip device achieves high throughput particle focusing without sheath fluid, enabling easier research applications.

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Last Updated: Jun 18, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

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Published on: July 10, 2016

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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Area of Science:

Biomedical Engineering
Microfluidics
Cell Biology

Background:

Commercial flow cytometers are bulky, expensive, and require specialized operation.
There is a significant need for affordable and portable single-cell analysis tools.

Purpose of the Study:

To develop a low-cost, on-chip flow cytometry system.
To demonstrate a sheath-less particle focusing technique using microfluidic principles.

Main Methods:

Utilized Dean coupled inertial microfluidics in a spiral microchannel for 3-D particle focusing.
Employed a laser-induced fluorescence (LIF) setup for particle detection.
Tested the system with 6 micrometer particles and SH-SY5Y neuroblastoma cells.

Main Results:

Achieved sheath-less, 3-D particle focusing solely through microchannel geometry.
Demonstrated a high throughput of 2,100 particles/sec.
Successfully performed cell counting using the developed microfluidic flow cytometer.

Conclusions:

The developed on-chip flow cytometry system offers a low-cost, portable alternative to commercial instruments.
The passive focusing principle and planar design facilitate integration with lab-on-a-chip systems.
This technology enhances accessibility of single-cell analysis for research.