[On the modified process of human hemoglobin based blood substitutes]

Area of Science:

• Biochemistry
• Biomaterials Science
• Chemical Engineering

Context:

• Hemoglobin (Hb) is a critical oxygen carrier.
• Modifications aim to improve Hb-based oxygen carrier (HBOC) properties.
• Pyridoxal 5-phosphate (PLP) and glutaric dialdehyde (GDA) are key reagents in Hb modification and polymerization.

Purpose:

• To compare the efficacy of modifying hemoglobin with PLP before and after polymerization with GDA.
• To evaluate the impact of GDA addition timing (before vs. after PLP) on Hb properties.
• To determine the optimal procedure for producing high-quality, cost-effective Hb-based products.

Summary:

• Purified hemoglobin modified with PLP and polymerized with GDA was investigated. No significant differences in molecular distribution, methemoglobin (MetHb) concentration, oxygen carrier capacity, P50, or spectra were observed between modifying before or after polymerization.
• Modification after polymerization significantly reduces PLP usage and cost, presenting a more economical approach.
• Adding GDA after PLP resulted in a more favorable P50 value compared to adding GDA before PLP, without compromising other critical properties.

Impact:

• The study identifies a more cost-effective and efficient method for producing modified hemoglobin products.
• The findings provide valuable insights for optimizing the production of hemoglobin-based oxygen carriers (HBOCs).
• This optimized procedure can facilitate advancements in HBOC research and large-scale production, potentially improving oxygen delivery therapies.