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Updated: Jun 18, 2026
Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
Published on: May 20, 2020
The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in
Anna Pistocchi1, Carmen G Feijóo, Pablo Cabrera
1Department of Biology, Università degli Studi di Milano, Milan, Italy. anna.pistocchi@unimi.it
Background:
The lateral line system in zebrafish is composed of a series of organs called neuromasts, which are distributed over the body surface. Neuromasts contain clusters of hair cells, surrounded by accessory cells.
Results:
In this report we describe zebrafish prox1 mRNA expression in the migrating primordium and in the neuromasts of the posterior lateral line. Furthermore, using an antibody against Prox1 we characterize expression of the protein in different cell types within neuromasts, and we show distribution among the supporting cells and hair cells.
Conclusion:
Functional analysis using antisense morpholinos indicates that prox1 activity is crucial for the hair cells to differentiate properly and acquire functionality, while having no role in development of other cell types in neuromasts.
