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[Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in
Hao Wang1, Peng Zhang, Chun-Ting Wang
1State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Objective:
To study on the expression pattern of a novel gene, Biot2-L, in mice and its primary bio-function on the development of testis.
Methods:
Bioinformatics analysis were applied to identify the structure characteristics and homologene of Biot2-L. RT-PCR and Real-time RT-PCR were used to analyze its expression profile in normal tissues and the time-dependent expression pattern during the testes sexual maturation.
Results:
The complete sequence of Biot2-L was obtained. A conserved domain-CCDC7 was detected and there were homolog proteins in advanced mammals with about 50% identity by bioinformatics analysis. The highly expression of Biot2-L in adult testis but low or absent expressed in other tissues was observed. A regular change of Biot2-L expression level during the testis development and spermatogonia was demonstrated by the results of Real-time RT-PCR. The mRNA transcript appeared from the fifth week of postnatal development, and the expression level increased up to adulthood, but decreased when get older.
Conclusion:
Biot2-L was highly expressed in adult testis and regular changed during the testis development, which suggested that the Biot2-L would be involved in the testis development and spermatogonia.
