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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 18, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Quantitative cell array screening to identify regulators of gene expression.

Pinay Kainth1, Brenda Andrews

  • 1Banting and Best Department of Medical Research, Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto, Ontario, Canada.

Briefings in Functional Genomics
|December 3, 2009
PubMed
Summary
This summary is machine-generated.

Reporter Synthetic Genetic Array (R-SGA) analysis advances functional genomics by enabling systematic discovery of gene regulators. This method aids in understanding complex gene expression pathways and transcriptional networks.

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Related Experiment Videos

Last Updated: Jun 18, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Genome-scale technologies have revolutionized gene function analysis.
  • Budding yeast serves as a model organism for developing functional genomics tools.
  • Understanding gene regulation is crucial for deciphering complex biological processes.

Purpose of the Study:

  • To review advances in reporter screening technology for identifying gene regulators.
  • To introduce the Reporter Synthetic Genetic Array (R-SGA) analysis methodology.
  • To highlight the potential of R-SGA for studying transcriptional regulatory networks.

Main Methods:

  • Reporter screening technology development.
  • Application of functional genomics tools in budding yeast.
  • Reporter Synthetic Genetic Array (R-SGA) analysis.

Main Results:

  • Systematic analysis of gene function is now feasible.
  • New functional genomics tools are being developed and applied.
  • R-SGA enables quantitative data collection on gene expression pathways.

Conclusions:

  • R-SGA analysis is a powerful approach for discovering trans-acting regulators.
  • This methodology facilitates a deeper understanding of transcriptional regulatory networks.
  • Advances in reporter screening pave the way for broader applications in higher eukaryotes.