Related Experiment Video
Updated: Jun 18, 2026

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
Published on: February 12, 2015
[Study on blood and buccal mucous related genes expression in the patients suffering from coal-burning arsenism]
1Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China.
Objective:
To observe the oxidative stress and skin disease related genes expression in cells exfoliated from buccal mucous and blood in patients with arseniasis caused by coal-burning pollution in Guizhou.
Methods:
Buccal and blood cell samples were collected from arsenicosis patients and healthy subjects residing in Guizhou. Total RNA was isolated for related genetic transcript analysis. Real-time PCR (RT-PCR) was employed to detect the change of related gene expression. Serum indicators of liver injury, such as serum alanine aminotransferase (ALT), were detected.
Results:
Serum levels of ALT in arsenic patients were significantly higher than those in controls (36.9 +/- 17.4 / 25.8 +/- 2.4, t = 0.019, P < 0.05) even when no symptom was found in these patients. Buccal K10 gene was overexpressed (3.6 +/- 0.9 / 1.8 +/- 0.7, t = 0.041, P < 0.05) and MT-1 expression was significantly lower (0.8 +/- 0.5 / 3.9 +/- 0.4, t = 0.028, P < 0.05) in arsenic patients than that in control while the expressions of other genes were the same as those of controls.
Conclusion:
The expressions of MT-1 and K10 gene in buccal mucous cells may be regarded as sensitive molecular markers for skin and hepatic pathologic changes in arsenic patients. Protection of liver function should be considered as an important step in arseniasis prevention and control.
More Related Videos
10:21Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification
Published on: September 20, 2024
09:33Visualizing Field Data Collection Procedures of Exposure and Biomarker Assessments for the Household Air Pollution Intervention Network Trial in India
Published on: December 23, 2022
Related Concept Videos
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
Medical History
Chronic Obstructive Pulmonary Disease-II: Pathophysiology
Chronic Inflammation
Blood Studies I: ABG and VBG
Arterial Blood Gas (ABG)
Arterial Blood Gas (ABG) studies are crucial for assessing the lungs' ability to supply oxygen and remove carbon dioxide, reflecting the patient's ventilation status. They also help understand the kidneys' capacity to reabsorb or...