Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lighting effects on optimal facial regions for remote heart rate measurement.

NPJ cardiovascular health·2026
Same author

Best-first search-based approach for mining top-k closed frequent itemsets from uncertain databases.

PloS one·2026
Same author

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same author

Image Restoration Learning via Noisy Supervision in Fourier Domain.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026
Same author

EventTracer: Fast Path Tracing-Based Event Stream Rendering.

IEEE transactions on visualization and computer graphics·2026
Same author

Seasonal Divergence between Microbiomes on Microplastics and Natural Particles Increases with Rising Water Temperatures in Urban Rivers.

Environmental science & technology·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Three-dimensional microscopy and sectional image reconstruction using optical scanning holography.

Edmund Y Lam1, Xin Zhang, Huy Vo

  • 1Imaging Systems Laboratory, Department of Electrical and Electronic Engineering, University of Hong Kong, Pokfulam Road, Hong Kong. elam@eee.hku.hk

Applied Optics
|December 4, 2009
PubMed
Summary
This summary is machine-generated.

Optical scanning holography (OSH) enables fast 3D microscopy by capturing entire volumes in a single scan. This study demonstrates effective sectional image reconstruction from OSH data of biological specimens.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Related Experiment Videos

Last Updated: Jun 18, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Area of Science:

  • Microscopy
  • Optical Imaging
  • Biomedical Engineering

Background:

  • Confocal microscopy offers high resolution but requires slow sequential scanning.
  • Achieving both fast acquisition and high axial resolution in 3D microscopy is challenging.
  • Optical Scanning Holography (OSH) presents a potential solution for rapid 3D volume capture.

Purpose of the Study:

  • To demonstrate sectional image reconstruction from experimental Optical Scanning Holography (OSH) data.
  • To validate the effectiveness of an inverse imaging sectioning technique for OSH.
  • To visualize reconstructed 3D microscopy data of biological specimens.

Main Methods:

  • Acquisition of a complete 3D volume as a digital hologram using OSH in a single scan.
  • Application of an inverse imaging sectioning technique to reconstruct individual axial planes.
  • Processing of experimental OSH data from biological samples.
  • Visualization of reconstructed sections using OSA Interactive Science Publishing software.

Main Results:

  • Successful sectional image reconstruction was achieved from experimental OSH data.
  • The inverse imaging sectioning technique effectively processed the digital hologram to yield distinct sections.
  • The method allowed for the visualization of the internal structures of biological specimens.

Conclusions:

  • OSH provides a viable method for rapid 3D microscopy, balancing acquisition speed and resolution.
  • Inverse imaging sectioning is an effective algorithm for reconstructing 3D data from OSH.
  • This approach facilitates detailed visualization of biological specimens in three dimensions.