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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 18, 2026

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
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High-efficient FLPo deleter mice in C57BL/6J background.

Yingjie Wu1, Chunxin Wang, Hui Sun

  • 1Endocrinology/Diabetes and Bone Disease Division, Mount Sinai School of Medicine, New York, New York, United States of America.

Plos One
|December 4, 2009
PubMed
Summary

We developed a new FLP deleter mouse line with enhanced recombination efficacy for conditional gene manipulation. This mouse model significantly improves gene targeting efficiency in mammalian genetic studies.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Mammalian Gene Function Studies

Background:

  • Conditional gene manipulation is crucial for studying mammalian gene functions.
  • Existing FLP deleter mouse lines exhibit limited recombination activity.
  • Additional site-specific recombinases like FLP and phi31 enhance gene manipulation flexibility.

Purpose of the Study:

  • To generate a novel FLP deleter mouse line with superior recombination efficacy.
  • To improve the efficiency of conditional gene targeting in mice.
  • To facilitate genetic studies of mammalian gene functions.

Main Methods:

  • Generation of a new FLP deleter mouse line using a mouse codon-optimized FLPo gene.
  • Utilizing a pure C57BJ/6 background for the transgenic mice.
  • Assessing recombination efficacy by observing the removal of FRT-flanked Neo cassette.

Main Results:

  • The novel FLP deleter mouse line demonstrated superior recombination efficacy compared to existing FLPe deleter mice.
  • 100% complete removal of the FRT-flanked Neo cassette was observed in F1 progeny.
  • The Neo cassette transmission to the F2 generation was independent of FLPo activity.

Conclusions:

  • The new FLP deleter mouse line significantly facilitates the gene targeting process.
  • This mouse model is valuable for advanced conditional gene manipulation in mammals.
  • The enhanced recombination efficacy offers a significant advantage for genetic research.