Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early detection of a severe autism spectrum disorder group in young children using eye-tracking measures.

Scientific reports·2026
Same author

Lipopolysaccharide uptake is augmented in lipopolysaccharide-tolerant mouse macrophage-like cells via increased CD14 expression.

FEBS open bio·2026
Same author

Animal models for the study of ADHD: the need for next-generation models.

Frontiers in psychiatry·2026
Same author

Copper deficiency impairs oligodendrocyte maturation and social behavior via mitophagy and mTOR suppression in ASD.

Science advances·2026
Same author

Safety and efficacy of a novel fecal microbiota transplantation method using hydrogen nanobubble water without antibiotics or bowel cleansing in children with autism spectrum disorder: an open-label, single-arm study demonstrating improvements in core and comorbidity symptoms.

Frontiers in pediatrics·2026
Same author

Analysis of Onset Timing and Risk Factors for Mesalazine-related Adverse Events Using the JADER Database.

In vivo (Athens, Greece)·2026

Related Experiment Video

Updated: Jun 18, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons
08:04

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

HMGA1a recognition candidate DNA sequences in humans.

Takayuki Manabe1, Taiichi Katayama, Masaya Tohyama

  • 1Division of Gene Expression Mechanism, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan. manabe@fujita-hu.ac.jp

Plos One
|December 4, 2009
PubMed
Summary

Researchers identified specific DNA sequences that bind to High Mobility Group Protein A1a (HMGA1a). These HMGA1a-binding sequences are crucial for understanding gene regulation in processes like cell growth and cancer development.

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Related Experiment Videos

Last Updated: Jun 18, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons
08:04

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • High Mobility Group Protein A1a (HMGA1a) is an architectural transcription factor involved in various biological processes.
  • HMGA1a binds AT-rich DNA sequences and interacts with gene promoters, but its precise binding motifs are not fully characterized.

Purpose of the Study:

  • To identify high-affinity DNA binding sequences for human HMGA1a.
  • To compare these identified sequences with known human promoter sequences.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) was employed to find HMGA1a-binding sequences.
  • Bioinformatic analysis (FASTA) was used to compare identified sequences against a database of human promoter sequences.

Main Results:

  • The study proposes "-(G/A)-G-(A/T)-(A/T)-A-T-T-T-" as candidate HMGA1a-binding sequences.
  • These candidate sequences demonstrated binding to native HMGA1a from SK-N-SH cells.
  • FASTA analysis revealed that each candidate sequence matched 500-900 known human promoter sequences, including some previously linked to HMGA1a.

Conclusions:

  • The identified candidate sequences provide valuable insights into HMGA1a's DNA binding preferences.
  • These findings are significant for further research into HMGA1a's roles in cell differentiation, growth, proliferation, and cancer pathogenesis.