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Related Experiment Video

Updated: Jun 18, 2026

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
10:45

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

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Long-term imaging in microfluidic devices.

Gilles Charvin1, Catherine Oikonomou, Frederick Cross

  • 1Laboratoire Joliot-Curie & Laboratoire de Physique, Ecole Normale Supérieure, Lyon, France.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2009
PubMed
Summary

Microfluidic devices enable long-term live-cell imaging, allowing researchers to track cell generations and gene expression changes over extended periods. This technology overcomes limitations of traditional methods for studying cellular processes.

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Area of Science:

  • Live-cell imaging
  • Microfluidics
  • Cell biology

Background:

  • Traditional live-cell imaging is limited by short experimental durations, typically one cell generation time.
  • Growing interest in applying microfluidic techniques to biological imaging has emerged.
  • Microfabrication offers design flexibility for powerful imaging assays.

Purpose of the Study:

  • To describe the fabrication and assembly of a microfluidic device for long-term imaging of proliferating cells.
  • To provide an example of using the device to monitor real-time gene expression changes in budding yeast.
  • To make long-term microfluidic cell imaging accessible to researchers without prior microfluidic experience.

Main Methods:

  • Fabrication of a microfluidic device using microfabrication techniques.

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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

Published on: September 10, 2018

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Last Updated: Jun 18, 2026

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
10:45

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

High-Resolution C. elegans Imaging Across All Larval Stages
07:49

High-Resolution C. elegans Imaging Across All Larval Stages

Published on: May 23, 2025

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics
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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

Published on: September 10, 2018

  • Assembly of the microfluidic device components.
  • Application of the device for long-term imaging of budding yeast, monitoring gene expression in real-time.

    • Main Results:

    • Development of a microfluidic device enabling long-term imaging of single cells over multiple generations.
    • Demonstration of real-time monitoring of gene expression changes in response to controlled environmental conditions.
    • Successful application in budding yeast, showcasing the utility of the microfluidic system.

    Conclusions:

    • Microfluidic systems provide a powerful platform for long-term, high-resolution live-cell imaging.
    • This technology facilitates the study of cellular responses to dynamic environmental changes and gene expression.
    • The described fabrication and application protocols aim to broaden the accessibility of microfluidic cell biology research.