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Related Experiment Video

Updated: Jun 7, 2025

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Published on: January 23, 2012

High-Throughput Measurement of Single-Fission Yeast Cell Volume Using Fluorescence Exclusion.

Larisa Venkova¹, Daniel García-Ruano^1,2, Akanksha Jain^1,2

¹Institute of Biochemistry and Cellular Genetics, CNRS UMR 5095 and University of Bordeaux, Bordeaux, France.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2024

Summary

Accurately measuring fission yeast cell volume is crucial for understanding cell biology. This study introduces a new method using fluorescence exclusion and microfluidics for precise, high-throughput cell volume determination in Schizosaccharomyces pombe.

Keywords:

Automated analysis Cell volume Deep-learning Fission yeast Fluorescence exclusion Live-cell imaging Microfluidics

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Area of Science:

Cell Biology
Microbiology
Biophysics

Background:

Cell volume is a critical determinant of cellular functions.
Accurate cell size measurement is essential for understanding cell cycle regulation.
Traditional methods using cell length in Schizosaccharomyces pombe provide inaccurate volume estimations.

Purpose of the Study:

To develop a robust, high-throughput method for direct single-cell volume measurement in fission yeast.
To overcome limitations of using cell length as a proxy for cell volume.
To enable detailed analysis of cell volume distribution in Schizosaccharomyces pombe populations.

Main Methods:

Utilized the Fluorescence Exclusion method.
Integrated microfluidics technologies for cell handling and measurement.
Developed a comprehensive protocol for S. pombe cell volume determination.

Main Results:

Established a reliable protocol for direct measurement of fission yeast cell volume.
Enabled accurate evaluation of cell volume and its distribution.
Overcame previous obstacles in high-throughput cell volume analysis.

Conclusions:

The developed method provides a significant advancement for studying cell size regulation in fission yeast.
Direct cell volume measurement offers a more accurate assessment than cell length alone.
This protocol facilitates deeper insights into the biological roles of cell volume.