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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

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Isolation and segregation of 44 microsatellite loci in the South African abalone Haliotis midae L

R Slabbert1, J Hepple, A Venter

  • 1Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa. rslabbrt@sun.ac.za

Animal Genetics
|December 5, 2009
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...