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Related Experiment Video

Updated: Jun 18, 2026

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

Lectin histochemistry for light and electron microscopy.

Su Ee Wong¹, Catherine E Winbanks, Chrishan S Samuel

¹Department of Nephrology, The Royal Melbourne Hospital, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|December 5, 2009

Summary

This summary is machine-generated.

Lectins offer a specific method for identifying glycoconjugates, improving cell characterization in tissues. This technique enhances understanding of kidney disease and tumor biology.

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Last Updated: Jun 18, 2026

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Area of Science:

Biochemistry
Histology
Cell Biology

Background:

Glycoconjugates are vital macromolecules found in all body tissues.
Their diverse carbohydrate structures help distinguish cell types and phenotypes.
The traditional Periodic Acid-Schiff (PAS) method lacks specificity for glycoconjugate analysis.

Purpose of the Study:

To highlight the specificity of plant-derived lectins for identifying carbohydrate moieties in glycoconjugates.
To present lectin binding as a superior alternative to PAS for detailed histological analysis.
To emphasize the utility of lectin histochemistry in understanding disease pathogenesis and cell biology.

Main Methods:

Utilizing the specific binding affinity of plant lectins to distinct carbohydrate structures on glycoconjugates.
Employing reporter molecules (biotin, gold) for visualizing lectin binding via light and electron microscopy.
Describing experimental protocols for microscopic examination of lectin binding.

Main Results:

Lectin binding provides precise characterization of cell types and phenotypes based on their glycoconjugate profiles.
This method offers valuable insights into the pathogenesis of kidney diseases.
It aids in analyzing cell surface carbohydrates in normal and neoplastic cells, and in blood group studies.

Conclusions:

Lectin-based histochemistry offers enhanced specificity and utility compared to traditional methods like PAS.
Lectin binding is crucial for detailed characterization of cell types, particularly in renal disease.
The described protocols facilitate advanced microscopic analysis of glycoconjugates in various biological contexts.