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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Photoluminescence: Applications

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Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

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Published on: October 28, 2018

Fluorescent probes and delivery methods for single-molecule experiments.

Konstantinos Lymperopoulos1, Alexander Kiel, Anne Seefeld

  • 1Cellnetworks Cluster and Institute for Physical Chemistry, Heidelberg University, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany. konstantinos.lymperopoulos@bioquant.uni-heidelberg.de

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|December 5, 2009
PubMed
Summary
This summary is machine-generated.

This study reviews novel fluorescent probes and delivery methods for advanced single-molecule experiments. It details probe customization for specific targets and cellular environments, enhancing spatial and temporal resolution.

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Last Updated: Jun 18, 2026

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14:16

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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

  • Biophysics
  • Chemical Biology
  • Spectroscopy

Background:

  • Single-molecule experiments demand higher spatial and temporal resolution.
  • Novel fluorescent probes and advanced spectroscopy techniques are crucial for this advancement.

Purpose of the Study:

  • To provide an overview of contributions to fluorescent probe development.
  • To discuss probe delivery methods for living cells.
  • To highlight probe customization for single-molecule spectroscopy.

Main Methods:

  • Review of fluorescent probe design and customization strategies.
  • Discussion of Förster resonance energy transfer (FRET) and photon-induced electron transfer (PET) for probe tuning.
  • Analysis of various probe delivery methods into living cells.

Main Results:

  • Customization of probes depends on target molecule, chemical state, and interaction type.
  • FRET and PET enhance probe customization capabilities.
  • Cell permeability and toxicity are critical considerations for in vivo experiments.

Conclusions:

  • Successful single-molecule studies require sophisticated probe design and tailored delivery methods.
  • Increased complexity in single-molecule studies yields richer biological insights.
  • Ongoing advancements in probes and delivery systems are vital for future research.