Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evolutionary Tree for All Bumblebee Species World-Wide Estimated by Combining Information from Fast-Evolving Genes, Slow-Evolving Genes, and Genomic Data (Apidae, <i>Bombus</i>).

Insects·2026

Same author

Metal-Organic Framework as a Bioorthogonal Catalyst for Gene Editing.

Journal of the American Chemical Society·2026

Same author

Dietary niche partitioning and convergent gut microbiota in sympatric <i>Vespa</i>.

Frontiers in microbiology·2026

Same author

Open-Loop, Barrier-Free, Continuous Langmuir-Blodgett-Based Multipass Multilayer Deposition.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Prevalence and host-migration patterns of Avian Haemosporidian Parasites in wild birds from Jeju Island, Republic of Korea.

Acta tropica·2026

Same author

Optimal 24-hour movement behaviour compositions across trimesters and risk of hypertensive disorders of pregnancy: the Pregnancy 24/7 cohort study.

British journal of sports medicine·2026

Same journal

Radical Cascades on Seawater Microdroplets Drive Atmospheric Mercury Oxidation.

Journal of the American Chemical Society·2026

Same journal

Superior Selective and Fast NH<sub>3</sub> Adsorption of Soft Porous MOF/Ionic Liquid Composites with Ordering Phase Transitions.

Journal of the American Chemical Society·2026

Same journal

Systematic Catalyst Variation for Improved Stereoselective Epoxide Polymerization: Subtle Modifications Resulting in Superior Efficiency.

Journal of the American Chemical Society·2026

Same journal

Deciphering the Halide Chemistry of Cl<sup>-</sup> and Br<sup>-</sup> in Enhancing Kinetics of Mg Plating/Stripping.

Journal of the American Chemical Society·2026

Same journal

Electrosynthesis of C<sub>6</sub> Chemicals by Propylene Oxidative Coupling on Au Surface.

Journal of the American Chemical Society·2026

Same journal

Statistical AI Enables Precise Screening of Multielement Catalysts.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Published on: September 21, 2020

Visualizing graphene based sheets by fluorescence quenching microscopy.

Jaemyung Kim¹, Laura J Cote, Franklin Kim

¹Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, USA.

Journal of the American Chemical Society

|December 8, 2009

Summary

This summary is machine-generated.

Researchers developed a new fluorescence quenching microscopy technique to easily visualize graphene based materials. This method allows for rapid sample evaluation and improved synthesis strategies without special substrates.

More Related Videos

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Published on: September 8, 2023

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Published on: May 17, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Published on: September 21, 2020

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Published on: September 8, 2023

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Published on: May 17, 2018

Area of Science:

Materials Science
Nanotechnology
Microscopy

Background:

Graphene based materials possess exceptional properties, driving significant research interest.
Current visualization techniques for graphene require specialized substrates, limiting flexibility.
A general, rapid imaging method is needed for evaluating and manipulating graphene sheets.

Purpose of the Study:

To develop a versatile fluorescence microscopy technique for visualizing graphene based sheets.
To overcome substrate limitations inherent in existing graphene imaging methods.
To enable rapid feedback for optimizing graphene synthesis and processing.

Main Methods:

Utilizing a fluorescence quenching mechanism by coating graphene sheets with a removable dye.
Employing fluorescence microscopy to observe the quenched emission from the dye.
Demonstrating visualization on various substrates and in solution.

Main Results:

Graphene, reduced graphene oxide, and graphene oxide sheets become highly visible.
The method provides good contrast for layer counting, independent of substrate type.
Visualization of suspended graphene sheets in solution was achieved.
The dye coating can be removed post-imaging without damaging the graphene sheets.

Conclusions:

Fluorescence quenching microscopy offers a flexible and general approach for graphene characterization.
This technique eliminates the need for specialized substrates, broadening applicability.
It provides immediate feedback, facilitating improved synthesis and processing of graphene materials.