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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

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Published on: November 2, 2009

Micro-fabricated fluorescence-activated cell sorter.

Sung Hwan Cho1, Chun H Chen, Frank S Tsai

  • 1University of California, San Diego, CA 92093, USA. scho@logroup.ucsd.edu

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|December 8, 2009
PubMed
Summary
This summary is machine-generated.

This study presents a high-throughput microfluidic cell sorter using an optofluidic waveguide and piezoelectric actuator. The device achieves efficient, automated cell sorting at high flow rates with excellent purity.

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Area of Science:

  • Microfluidics
  • Optofluidics
  • Biotechnology

Background:

  • Cell sorting is crucial for biological research and diagnostics.
  • Existing methods face limitations in throughput, sensitivity, or automation.

Purpose of the Study:

  • To develop a high-sensitivity, high-throughput microfluidic cell sorting device.
  • To integrate piezoelectric actuation and optofluidic waveguides on a single chip.
  • To implement a real-time control system for automated sorting.

Main Methods:

  • Microfabrication of a cell sorting chip with integrated piezoelectric actuator and optofluidic waveguide.
  • Development of a field-programmable-gate-array (FPGA) embedded real-time control loop system.
  • Utilizing a Teflon AF coated liquid core waveguide (LCW) for enhanced fluorescence detection sensitivity.

Main Results:

  • Achieved high throughput (>1kHz) sorting at high flow rates (approx. 10 cm/sec).
  • Demonstrated high purity sorting with no false sorting.
  • Reported sorting efficiency of approximately 70% for fluorescent beads.

Conclusions:

  • The developed microfluidic cell sorter offers high sensitivity and throughput.
  • The integrated optofluidic waveguide and real-time control system enable efficient automated sorting.
  • This technology has potential applications in various biological and medical fields.