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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Jun 18, 2026

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Tablet--next generation sequence assembly visualization.

Iain Milne¹, Micha Bayer, Linda Cardle

¹Genetics Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK.

Bioinformatics (Oxford, England)

|December 8, 2009

Summary

This summary is machine-generated.

Tablet is a powerful graphical viewer for analyzing next-generation sequence data. This lightweight software efficiently handles millions of reads for assembly and alignment visualization.

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Area of Science:

Bioinformatics
Genomics
Computational Biology

Background:

Next-generation sequencing (NGS) technologies generate vast amounts of data.
Efficient visualization tools are crucial for analyzing large-scale genomic datasets.
Existing tools may struggle with the scale and complexity of modern sequence assemblies.

Purpose of the Study:

To introduce Tablet, a novel graphical viewer for sequence assemblies and alignments.
To provide a high-performance, memory-efficient solution for visualizing large genomic datasets.
To enable researchers to easily navigate and analyze complex sequence data.

Main Methods:

Tablet supports various input assembly formats.
It offers packed and stacked views for data visualization.
The software is designed for multi-core processors and memory efficiency.

Main Results:

Tablet provides high-quality visualizations of sequence assemblies and alignments.
It allows instant access and navigation to specific genomic regions.
The viewer can handle assemblies with millions of reads on standard desktop machines.

Conclusions:

Tablet is a lightweight, high-performance graphical viewer for next-generation sequence assemblies and alignments.
Its efficient design and visualization capabilities make it suitable for large-scale genomic data analysis.
Tablet is freely available for major operating systems.