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Published on: September 15, 2021
Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations
Nobuhisa Watanabe1, Yozo Takasaki, Chika Sato
1Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Japan. nobuhisa@nagoya-u.jp
Abstract:
The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.
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