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Interleukin-8 induces neutrophil transendothelial migration
W B Smith1, J R Gamble, I Clark-Lewis
1Division of Human Immunology, Institute of Medical and Veterinary Sciences, Adelaide, South Australia.
Immunology
|January 1, 1991
Summary
Interleukin-8 (IL-8) significantly boosts neutrophil adhesion and their movement across endothelial cells. This supports IL-8's key role in neutrophil accumulation at inflammation sites.
Area of Science:
- Immunology
- Cell Biology
Background:
- Interleukin-8 (IL-8) is a key signaling molecule.
- Neutrophils play a critical role in inflammatory responses.
Purpose of the Study:
- To investigate the role of Interleukin-8 (IL-8) in human neutrophil adhesion and transendothelial migration.
- To understand IL-8's function in inflammatory processes.
Main Methods:
- Chemically synthesized IL-8 was used to study human neutrophil behavior.
- Experiments involved assessing neutrophil adhesion to plastic and endothelial cells.
- A two-compartment model with endothelial monolayers was employed to measure transendothelial migration.
Main Results:
- IL-8 enhanced neutrophil adhesion to both plastic and endothelial cells (unstimulated and TNF-stimulated).
- Chemotactic IL-8 stimulation induced significant neutrophil transmigration (87.4%) across endothelial monolayers.
- Tumor necrosis factor (TNF) preincubation also induced transmigration, showing additive effects with IL-8.
Conclusions:
- IL-8 effectively stimulates neutrophil movement across endothelial barriers in vitro.
- Increased endothelial permeability was observed during maximal IL-8-induced transmigration.
- These findings highlight IL-8's central role in neutrophil recruitment to inflammatory sites in vivo.